The cytoskeleton, composed primarily of microtubules and actin filaments, plays a crucial role in maintaining cellular architecture, regulating intracellular transports and supporting dynamic processes. Beyond their structural roles, cytoskeletal proteins also contribute to electrostatic regulation and can act as pathways for intracellular bioelectric signalling. Understanding how external physical stimuli modulate these proteins is therefore of biological and therapeutic interest. This thesis investigated the effects of electromagnetic field (EMF) stimulation on tubulin and G-actin using complementary biophysical approaches. Dynamic Light Scattering (DLS) was used to monitor hydrodynamic diameter and zeta potential, turbidity assays assessed polymerization dynamics, Raman spectroscopy analysed molecular vibrations and conductivity measurements proved electrostatic and charge transport properties.