The protein corona, caused by nanoparticle-protein interaction on the surface of nanoparticles, has long been studied due to its ability to modulate the particle’s biological properties. It is a dynamic layer of proteins that adsorb onto nanoparticles, influencing their structural characteristics and functions. There are two types of protein corona: hard corona, composed of tightly bound and hardly dissociable proteins, and soft corona, composed of weakly bound proteins with more frequent exchange. Lipoproteins, whose size ranges from 10 to 1000 nm, are an important family of nanoparticles; nevertheless, the study of the protein corona on lipoproteins remains largely unexplored. This thesis aims to investigate the protein corona composition of low-density lipoproteins (LDL) isolated through different extraction protocols and purification methods.