According to the World Health Organization, each year approximately 1.3 million people die as a result of road traffic collisions. More than half of all road traffic fatalities are among vulnerable road users which include pedestrians, cyclists, and motorcyclists. The aim of this study is to identify and evaluate methods for the spatio-temporal analysis of road collisions involving vulnerable road users (VRU), using a crash database of the Turin municipality for the years 2006-2020. In the urban road network, collisions tend to cluster at specific points, also called hotspots or hazardous road locations (HRL).Therefore, it is of fundamental importance to identify them in order to (i) define their dependence to road geometric, environmental and operational factors, and (ii) intervene with safety countermeasures.