This thesis is performed in the contex of Nat4more project in collaboration with the Innovation Center of Island. In Island it was studied the behavior of chitosan DD50 film on hydroxyapatite, SCNA bioglass and titanium substrate before and after packaging, sterilization and 6 month storages. It was done Raman, SEM, microscope analysis of the samples and also UV analysis before and after incubation 24h, 3d and 7d at 37°C in distilled water. This is done to see the chitosan release in each substrates and the impact of packaging, sterilization and storage on chitosan coating. In adddition it is performed different chitosan coating on Ti and HA substrate with chitosan 50DD and 70DD, also with TPP crosslinking 15%w/v.